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Objective To investigate the role of Wnt/β-catenin signaling pathway on hypoxia induced proliferation and invasion in os -teosarcoma SaOS-2 cell line and its mechanism.Methods Cultured SaOS-2 cells were treated with normoxia(21%O2)and hypoxia (1%O2)for 24 hours respectively.The proliferation rate were detected by CCK-8;the cell invasion ability were detected by Transwell inva-sion test;the mRNA and protein expression levels of Wnt/β-catenin were detected by RT-PCR and Western blot.Furthermore,β-catenin siR-NA was used to investigate the role of β-catenin on hypoxia induced proliferation and invasion.Results Hypoxia microenvironment signifi-cantly increased the proliferation of osteosarcoma SaOS-2 cell line and enhanced cell invasion potentiality.The mRNA and protein level of Wnt/β-catenin was significantly increased by hypoxia treatment.Targeted kept Wnt/β-catenin could obviously attenuated the proliferation and invasion ability of osteosarcoma SaOS-2 cell line induced by hypoxia.Conclusion Hypoxia promote the proliferation and invasion of osteosarco-ma SaOS-2 cell line by obviously activating Wnt/β-catenin signaling pathway.
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Objective To investigate the application of thoracoscope combined with mechanical ventilation in elderly patients with chest trauma complicated by acute respiratory distress syndrome(ARDS).Methods Selected 49 elderly patients with chest trauma complicated by ARDS who were admitted into our hospital from Jan.2015 to Jun.2017 as the study objects.And divided these patients into the control group (n=25)and the observation group(n=24)upon the different treatment method.Patients of the control group underwent conventional thora-cotomy combined with mechanical ventilation,while patients of the control group underwent the microinvasive video -assisted thoracoscopic surgery(VATS)combined with mechanical ventilation.Post-operative incision size,duration of operation,intraoperative bleeding,severity of pain 5 days after surgery,drainage duration,patients with post-operative complication,and hospital stays after operation of all patients were e-valuated.Compared and analyzed the changes of pulmonary function before surgery and 1 week after surgery, as well as the VAS score and anxiety and depression of patients before and after surgery.Results The postoperative incision size,VAS score and operation time in the ob-servation group were significantly better those of the control group,and the difference was statistically significant(P<0.05).The postopera-tive complications of the observation group were significantly lower than that in the control group,and the difference was statistically signifi-cant(χ2=4.75,P<0.05).One week after treatment,the pulmonary function index of the observation group were obviously superior to the control group with statistically significant difference(P<0.05).And the improvement degree of anxiety and depression was better in the ob-servation group compared to the control group(P<0.05).Conclusion Compared to traditional thoracotomy,reasonable VATS will help to decrease the intra-operative bleeding,duration of operation,post-operative hospital stays,and number of complications after surgery.What is more,it can improve pulmonary function of elderly patients.In addition,it can relief the degree of anxiety and depression of patients after op-eration,which is better than ones accepting traditional thoracotomy.
ABSTRACT
<p><b>OBJECTIVE</b>To discuss the influence of intensive insulin therapy on insulin resistance of patients with severe burn or trauma.</p><p><b>METHODS</b>Sixty patients with severe burn or trauma hospitalized in the Third People's Hospital of Chongqing or Southwest Hospital of the Third Military Medical University from January 2010 to December 2011 were randomly divided into intensive insulin therapy group (IT, treated with intensive insulin therapy to control the blood glucose to the level of 6.0-8.0 mmol/L) and control group (C, treated with routine therapy) according to the paired grouping method, with 30 patients in each group. Before treatment and on post treatment day (PTD) 1, 3, 7, 10, 14, the levels of fasting blood glucose and fasting plasma insulin were determined. Insulin resistance index and β-cell function index were calculated using homeostasis model assessment. Data were processed with t test, analysis of variance, and LSD test.</p><p><b>RESULTS</b>On PTD 1, 3, 7, 10, levels of fasting blood glucose in group IT [(6.8 ± 1.4), (6.7 ± 1.3), (5.8 ± 1.9), (5.4 ± 1.6) mmol/L] were significantly lower than those of group C [(14.8 ± 4.9), (12.7 ± 3.7), (7.7 ± 1.9), (6.6 ± 1.3) mmol/L, with t values respectively 12.453, 11.386, 5.563, 4.731, P < 0.05 or P < 0.01]. On PTD 3, 7, levels of fasting insulin in group IT [(14 ± 5), (10 ± 3) mU/L] were significantly lower than those of group C [(16 ± 4), (13 ± 4) mU/L, with t values respectively 4.212, 4.364, P values below 0.05]. Levels of fasting blood glucose and fasting insulin in the two groups at each time point were statistically significantly different from those before treatment (with P values below 0.01), except for the level of fasting blood glucose on PTD 3. On PTD 1, 3, 7, 10, levels of insulin resistance index in group IT (1.60 ± 0.80, 1.46 ± 0.70, 0.96 ± 0.21, 0.90 ± 0.23) were significantly lower than those in group C (2.15 ± 1.35, 2.21 ± 1.21, 1.50 ± 0.95, 1.17 ± 0.66, with t values respectively 8.316, 10.607, 7.825, 5.217, P < 0.05 or P < 0.01). Levels of insulin resistance index of patients in the two groups at each time point after treatment were significantly lower than those before treatment (with P values below 0.01). On PTD 1, 3, 7, levels of β-cell function index in group IT (4.6 ± 2.9, 4.5 ± 3.3, 4.5 ± 3.6) were significantly higher than those in group C (3.4 ± 2.5, 3.6 ± 2.2, 4.2 ± 2.5, with t values respectively 8.243, 7.914, 4.338, P < 0.05 or P < 0.01). Levels of β-cell function index in group C on PTD 1 and 3 were significantly lower than that before therapy (with P values below 0.05).</p><p><b>CONCLUSIONS</b>Intensive insulin therapy can alleviate insulin resistance of patients with severe burn or trauma.</p>